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AllCells LLC fresh human bone marrow aspirates
Fresh Human Bone Marrow Aspirates, supplied by AllCells LLC, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/fresh+human+bone+marrow+aspirates/human+bone+marrow+aspirates/pm37752338-102-11-22
Average 90 stars, based on 1 article reviews
fresh human bone marrow aspirates - by Bioz Stars, 2026-09
90/100 stars

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Isolation:

Article Title: A Combination of Atorvastatin and Aspirin Enhances the Pro-Regenerative Interactions of Marrow Stromal Cells and Stroke-Derived Monocytes In Vitro
Article Snippet: .. MSCs were isolated from commercially available fresh human bone marrow aspirates of a 34 year old male (AllCells, Alameda, CA) using density centrifugation and plastic adherence as previously described ( ). ..

Article Title: Inflammation-Stimulated Mesenchymal Stromal Cell-Derived Extracellular Vesicles Attenuate Inflammation.
Article Snippet: .. MATERIALS AND METHODS Isolation and culture of human mesenchymal stromal cells (hMSCs) hMSCs were isolated from commercially available fresh human bone marrow aspirates of a 34 year old female (AllCells, Alameda, CA) using density centrifugation and plastic adherence as previously described _ENREF_1[18]. ..

Article Title: Clinical Cellular Therapeutics Accelerate Clot Formation
Article Snippet: Upon reaching desired confluency, the medium was discarded; the cultures were washed with PBS and the adherent cells harvested with 0.25% trypsin/1 mM EDTA for 5 minutes at 37°C and frozen at 10 6 cells per ml in a cryosolution containing 10% DMSO (Cryostor CS10, Stem Cell Technologies, Vancouver, BC, Canada) for subsequent experiments. .. Bone marrow derived MSCs (BM MSCs) were isolated from commercially available fresh human bone marrow aspirates (AllCells, Emeryville, CA) or obtained from fresh bone marrow through the approved IRB protocol HSC‐MS‐08‐0393 and expanded following established procedures . .. Briefly, BM MSCs were cultured in complete culture medium that consisted of alpha‐minimal essential medium (Life Technologies, Grand Island, NY), 17% fetal bovine serum (FBS; lot‐selected for rapid growth of MSC; Atlanta Biologicals, Norcross, GA), 100 units/ml penicillin (Thermo Fisher Scientific, Waltham, MA), 100 mg/ml streptomycin (Life Technologies), and 2 mM l ‐glutamine (Thermo Fisher Scientific, Waltham, MA).

Article Title: Extracellular Vesicles Attenuate Nitrofen-Mediated Human Pulmonary Artery Endothelial Dysfunction: Implications for Congenital Diaphragmatic Hernia.
Article Snippet: Th is p a p er h as b ee n p ee rre vi ew ed a n d a cc e p te d f o r p u b lic at io n , b u t h as y et t o u n d er go c o p ye d it in g a n d p ro o f co rr ec ti o n . T h e fi n al p u b lis h ed v er si o n m ay d if fe r fr o m t h is p ro o f. Extracellular vesicles attenuate nitrofen-mediated human pulmonary artery endothelial dysfunction: implications for congenital diaphragmatic hernia

Article Title: Extracellular vesicles influence the pulmonary arterial extracellular matrix in congenital diaphragmatic hernia.
Article Snippet: Extracellular vesicles influence the pulmonary arterial extracellular matrix in congenital diaphragmatic hernia Madeline N. Monroe, BS, Siqin Zhaorigetu, PhD, Vikas S. Gupta, MD, Di Jin, Katelyn D. Givan, Alexander L. Curlyo, Scott D. Olson, PhD, Charles S. Cox, Jr, MD, Ana Segura, MD, L. Maximilian Buja, MD, K. Jane Grande-Allen, PhD and Matthew T. Harting, MD, MS § Department of Bioengineering, Rice University, Houston, TX, USA † Department of Pediatric Surgery, McGovern Medical School at the University of Texas Health Science Center and Children’s Memorial Hermann Hospital, Houston, TX, USA

Article Title: Medications for Hypertension Change the Secretome Profile from Marrow Stromal Cells and Peripheral Blood Monocytes
Article Snippet: .. MSCs were isolated from commercially available fresh human bone marrow aspirates (AllCells, Alameda, CA) using density centrifugation and plastic adherence as previously described [ ]. ..

Article Title: Validation and characterization of a novel blood-brain barrier platform for investigating traumatic brain injury.
Article Snippet: .. Bone marrow derived MSCs (BM MSCs) were isolated from commercially available fresh human bone marrow aspirates from a 28 yr old male (AllCells, Alameda, CA) using density centrifugation and plastic adherence as previously described. ..

Centrifugation:

Article Title: A Combination of Atorvastatin and Aspirin Enhances the Pro-Regenerative Interactions of Marrow Stromal Cells and Stroke-Derived Monocytes In Vitro
Article Snippet: .. MSCs were isolated from commercially available fresh human bone marrow aspirates of a 34 year old male (AllCells, Alameda, CA) using density centrifugation and plastic adherence as previously described ( ). ..

Article Title: Inflammation-Stimulated Mesenchymal Stromal Cell-Derived Extracellular Vesicles Attenuate Inflammation.
Article Snippet: .. MATERIALS AND METHODS Isolation and culture of human mesenchymal stromal cells (hMSCs) hMSCs were isolated from commercially available fresh human bone marrow aspirates of a 34 year old female (AllCells, Alameda, CA) using density centrifugation and plastic adherence as previously described _ENREF_1[18]. ..

Article Title: Extracellular Vesicles Attenuate Nitrofen-Mediated Human Pulmonary Artery Endothelial Dysfunction: Implications for Congenital Diaphragmatic Hernia.
Article Snippet: Th is p a p er h as b ee n p ee rre vi ew ed a n d a cc e p te d f o r p u b lic at io n , b u t h as y et t o u n d er go c o p ye d it in g a n d p ro o f co rr ec ti o n . T h e fi n al p u b lis h ed v er si o n m ay d if fe r fr o m t h is p ro o f. Extracellular vesicles attenuate nitrofen-mediated human pulmonary artery endothelial dysfunction: implications for congenital diaphragmatic hernia

Article Title: Extracellular vesicles influence the pulmonary arterial extracellular matrix in congenital diaphragmatic hernia.
Article Snippet: Extracellular vesicles influence the pulmonary arterial extracellular matrix in congenital diaphragmatic hernia Madeline N. Monroe, BS, Siqin Zhaorigetu, PhD, Vikas S. Gupta, MD, Di Jin, Katelyn D. Givan, Alexander L. Curlyo, Scott D. Olson, PhD, Charles S. Cox, Jr, MD, Ana Segura, MD, L. Maximilian Buja, MD, K. Jane Grande-Allen, PhD and Matthew T. Harting, MD, MS § Department of Bioengineering, Rice University, Houston, TX, USA † Department of Pediatric Surgery, McGovern Medical School at the University of Texas Health Science Center and Children’s Memorial Hermann Hospital, Houston, TX, USA

Article Title: Medications for Hypertension Change the Secretome Profile from Marrow Stromal Cells and Peripheral Blood Monocytes
Article Snippet: .. MSCs were isolated from commercially available fresh human bone marrow aspirates (AllCells, Alameda, CA) using density centrifugation and plastic adherence as previously described [ ]. ..

Article Title: Validation and characterization of a novel blood-brain barrier platform for investigating traumatic brain injury.
Article Snippet: .. Bone marrow derived MSCs (BM MSCs) were isolated from commercially available fresh human bone marrow aspirates from a 28 yr old male (AllCells, Alameda, CA) using density centrifugation and plastic adherence as previously described. ..

Derivative Assay:

Article Title: Clinical Cellular Therapeutics Accelerate Clot Formation
Article Snippet: Upon reaching desired confluency, the medium was discarded; the cultures were washed with PBS and the adherent cells harvested with 0.25% trypsin/1 mM EDTA for 5 minutes at 37°C and frozen at 10 6 cells per ml in a cryosolution containing 10% DMSO (Cryostor CS10, Stem Cell Technologies, Vancouver, BC, Canada) for subsequent experiments. .. Bone marrow derived MSCs (BM MSCs) were isolated from commercially available fresh human bone marrow aspirates (AllCells, Emeryville, CA) or obtained from fresh bone marrow through the approved IRB protocol HSC‐MS‐08‐0393 and expanded following established procedures . .. Briefly, BM MSCs were cultured in complete culture medium that consisted of alpha‐minimal essential medium (Life Technologies, Grand Island, NY), 17% fetal bovine serum (FBS; lot‐selected for rapid growth of MSC; Atlanta Biologicals, Norcross, GA), 100 units/ml penicillin (Thermo Fisher Scientific, Waltham, MA), 100 mg/ml streptomycin (Life Technologies), and 2 mM l ‐glutamine (Thermo Fisher Scientific, Waltham, MA).

Article Title: Validation and characterization of a novel blood-brain barrier platform for investigating traumatic brain injury.
Article Snippet: .. Bone marrow derived MSCs (BM MSCs) were isolated from commercially available fresh human bone marrow aspirates from a 28 yr old male (AllCells, Alameda, CA) using density centrifugation and plastic adherence as previously described. ..

other:

Article Title: Alternative splicing links histone modifications to stem cell fate decision
Article Snippet: After reaching ~ 70% confluence, the adherent cells were harvested at the passage 1 (P1) hAT-MSC/AEY239. hBM-MSCs were isolated from commercially available fresh human bone marrow (hBM) aspirates (AllCells, Emeryville, CA, USA) and expanded following a standard protocol [ ].



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Fresh Human Bone Marrow Aspirates, supplied by Lonza, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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A Schematic of <t>human</t> <t>bone</t> <t>marrow</t> stromal cells (hBMSCs) isolation, expansion, and differentiation into human osteoblasts (hOBs) on DBP. After 2 weeks of remineralization in osteogenic differentiation medium, humanized RdBP was prepared by decellularization. B (i) hOBs adhere following the collagen fiber orientation on DBP but randomly on TCP. (ii) Alizarin red mineral staining after hOB culture on DBP and TCP. (iii) Time-course quantitative measurement of mineral deposition by fluorescent calcein staining ( n = 6 independent samples). C (i) Schematic of human CD14 + mononuclear cells (hCD14 + MNCs) isolation and expansion from peripheral blood using magnetic-activated cell sorting system. (ii) Brightfield images of ex vivo expanded hCD14 + cells for 1 week culture with hM-CSF. Representative images from 3 independent experiments. (iii) hCD14 + cells cultured on TCP, HP, and RdBP for 2 weeks with hM-CSF and hRANKL to induce hOC differentiation. D (i) Representative images of hOCs on TCP, HP, and RdBP. (green: phalloidin, blue: DAPI) (ii) Characterization of hOC sizes on TCP, HP, and RdBP after 2 weeks of the stimulated culture ( n = 20 cells from three independent experiments). E (i) Representative time-course fluorescent images of calcein-coated mineral resorption by hOCs on control, OS680-, and Zoledronate (Zol)-treated RdBP. (ii) Time-course quantitative monitoring of resorption areas. ( n = 3 independent samples) Inner panel images show mineral resorption (purple: OS680). F (i) Representative fluorescent images of hOCs on control, OS680-, and Zol-treated RdBP (green: phalloidin, blue: DAPI). (ii) Normalized hOC density at the end of 2 weeks culture ( n = 5 independent samples). The data are presented as mean ± standard deviation. P -values are derived from unpaired two-tailed t -tests. ns: not significant ( p > 0.05). Related source data are provided as a Source Data file.
Fresh Human Bone Marrow Aspirate, supplied by AllCells LLC, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


A Schematic of human bone marrow stromal cells (hBMSCs) isolation, expansion, and differentiation into human osteoblasts (hOBs) on DBP. After 2 weeks of remineralization in osteogenic differentiation medium, humanized RdBP was prepared by decellularization. B (i) hOBs adhere following the collagen fiber orientation on DBP but randomly on TCP. (ii) Alizarin red mineral staining after hOB culture on DBP and TCP. (iii) Time-course quantitative measurement of mineral deposition by fluorescent calcein staining ( n = 6 independent samples). C (i) Schematic of human CD14 + mononuclear cells (hCD14 + MNCs) isolation and expansion from peripheral blood using magnetic-activated cell sorting system. (ii) Brightfield images of ex vivo expanded hCD14 + cells for 1 week culture with hM-CSF. Representative images from 3 independent experiments. (iii) hCD14 + cells cultured on TCP, HP, and RdBP for 2 weeks with hM-CSF and hRANKL to induce hOC differentiation. D (i) Representative images of hOCs on TCP, HP, and RdBP. (green: phalloidin, blue: DAPI) (ii) Characterization of hOC sizes on TCP, HP, and RdBP after 2 weeks of the stimulated culture ( n = 20 cells from three independent experiments). E (i) Representative time-course fluorescent images of calcein-coated mineral resorption by hOCs on control, OS680-, and Zoledronate (Zol)-treated RdBP. (ii) Time-course quantitative monitoring of resorption areas. ( n = 3 independent samples) Inner panel images show mineral resorption (purple: OS680). F (i) Representative fluorescent images of hOCs on control, OS680-, and Zol-treated RdBP (green: phalloidin, blue: DAPI). (ii) Normalized hOC density at the end of 2 weeks culture ( n = 5 independent samples). The data are presented as mean ± standard deviation. P -values are derived from unpaired two-tailed t -tests. ns: not significant ( p > 0.05). Related source data are provided as a Source Data file.

Journal: Nature Communications

Article Title: Functional and analytical recapitulation of osteoclast biology on demineralized bone paper

doi: 10.1038/s41467-023-44000-9

Figure Lengend Snippet: A Schematic of human bone marrow stromal cells (hBMSCs) isolation, expansion, and differentiation into human osteoblasts (hOBs) on DBP. After 2 weeks of remineralization in osteogenic differentiation medium, humanized RdBP was prepared by decellularization. B (i) hOBs adhere following the collagen fiber orientation on DBP but randomly on TCP. (ii) Alizarin red mineral staining after hOB culture on DBP and TCP. (iii) Time-course quantitative measurement of mineral deposition by fluorescent calcein staining ( n = 6 independent samples). C (i) Schematic of human CD14 + mononuclear cells (hCD14 + MNCs) isolation and expansion from peripheral blood using magnetic-activated cell sorting system. (ii) Brightfield images of ex vivo expanded hCD14 + cells for 1 week culture with hM-CSF. Representative images from 3 independent experiments. (iii) hCD14 + cells cultured on TCP, HP, and RdBP for 2 weeks with hM-CSF and hRANKL to induce hOC differentiation. D (i) Representative images of hOCs on TCP, HP, and RdBP. (green: phalloidin, blue: DAPI) (ii) Characterization of hOC sizes on TCP, HP, and RdBP after 2 weeks of the stimulated culture ( n = 20 cells from three independent experiments). E (i) Representative time-course fluorescent images of calcein-coated mineral resorption by hOCs on control, OS680-, and Zoledronate (Zol)-treated RdBP. (ii) Time-course quantitative monitoring of resorption areas. ( n = 3 independent samples) Inner panel images show mineral resorption (purple: OS680). F (i) Representative fluorescent images of hOCs on control, OS680-, and Zol-treated RdBP (green: phalloidin, blue: DAPI). (ii) Normalized hOC density at the end of 2 weeks culture ( n = 5 independent samples). The data are presented as mean ± standard deviation. P -values are derived from unpaired two-tailed t -tests. ns: not significant ( p > 0.05). Related source data are provided as a Source Data file.

Article Snippet: Fresh human bone marrow aspirate (50 mL) was purchased from Lonza (age 25–45 male and female donors).

Techniques: Isolation, Staining, FACS, Ex Vivo, Cell Culture, Control, Standard Deviation, Derivative Assay, Two Tailed Test